Description | This Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. With a target-specific guide RNA (sgRNA), it can bind to a specific region of DNA without cutting it. This vector provides an in-frame downstream multiple cloning site, allowing users to attach any protein of interest to the C-terminus of dCas9. The resulting construct will also carry a C-terminal nuclear localization signal. |
---|---|
SKU | K014 |
System | Lentiviral Vector |
Vector Map | pLenti-EF1a-dCas9-Cloning |
Unit quantity | 10 µg |
Promoter | EF1a |
Cas Type | Null Mutant |
Cas Origin | spCas9 |
Cas9 Expression | Constitutive Cas9 |
Shipping Conditions | Shipped at ambient temperature |
Bacterial Selection | Kanamycin |
Format | Vector |
Appearance | Liquid |
Quality Control | Restriction Enzyme Digest and Sequencing |
Shelf Life | 1 year (when stored at -20°C or below) |
Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
abm提供了范围广泛的单个酶和试剂盒,可用于制备RNA和DNA,以便在下一代全基因组测序和文库构建中的后续应用。凭借在蛋白质工程领域的丰富经验和长处,abm自豪地坚持其承诺价值,质量和可靠性的产品。
多年来,我们的科学家为PCR相关应用设计了最优质的酶和最先进,最复杂的配方。下表概述了我们的DNA聚合酶的特征和形式。
特性 | 中号ega ˚F 我 ™˚F idelity | 乙拉斯 Ť aq ™ | Ħ ot小号挞 Ť 水溶液 |
---|---|---|---|
校对 | 是 | 没有 | 没有 |
保真度(相对于本地Taq) | 1300倍 | 1倍 | 1倍 |
特异性 | ●●●● | ●●● | ●●●● |
延伸速度 | 20-30秒/ kb | 15秒/ kb | 60秒/ kb |
目标长度 | 15-20 kb | 10-15 kb | 6 kb |
适用于TA克隆 | 没有 | 是 | 是 |
格式 | 酵素(G896) 主混合物(G897) | 酵素(G895) 万事达(G984) | 酶(G011 / G039) 主混合物 (G906) |
专刊 | 保真度和灵敏度 | 快速而强大 | 高特异性 |