| Description | dCas9-KRAB is used for CRISPR gene repression (CRISPRi). The Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. In this construct, dCas9 carries a C-terminal Krüppel associated box (KRAB) domain. By targeting dCas9-KRAB to a promoter region, users will be able to specifically repress expression of virtually any gene of interest. This dCas9-KRAB lentiviral vector may be used for either non-viral transfection, or packaged into lentivirus for stable integration of dCas9-KRAB into host cells. Use this dCas9-KRAB lentiviral vector in conjunction with a CRISPRi sgRNA lentiviral vector designed specifically for CRISPR repression. |
|---|---|
| SKU | K203 |
| System | Lentiviral Vector |
| Vector Map | pLenti-EF1a-dCas9-KRAB |
| Unit quantity | 10 µg |
| Promoter | EF1a |
| Cas Type | Null Mutant |
| Cas Origin | spCas9 |
| Cas9 Expression | Constitutive Cas9 |
| Shipping Conditions | Shipped at ambient temperature |
| Bacterial Selection | Kanamycin |
| Format | Vector |
| Appearance | Liquid |
| Quality Control | Restriction Enzyme Digest and Sequencing |
| Shelf Life | 1 year (when at -20°C or below) |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
- Dai, X., Chen, X., Hakizimana, O., & Mei, Y. "Genetic interactions between ANLN and KDR are prognostic for breast cancer survival" Oncology Reports. : (2019).
abm提供了范围广泛的单个酶和试剂盒，可用于制备RNA和DNA，以便在下一代全基因组测序和文库构建中的后续应用。凭借在蛋白质工程领域的丰富经验和长处，abm自豪地坚持其承诺价值，质量和可靠性的产品。
产品信息
多年来，我们的科学家为PCR相关应用设计了最优质的酶和最先进，最复杂的配方。下表概述了我们的DNA聚合酶的特征和形式。
| 特性 | 中号ega ˚F 我 ™˚F idelity | 乙拉斯 Ť aq ™ | Ħ ot小号挞 Ť 水溶液 |
|---|---|---|---|
| 校对 | 是 | 没有 | 没有 |
| 保真度（相对于本地Taq） | 1300倍 | 1倍 | 1倍 |
| 特异性 | ●●●● | ●●● | ●●●● |
| 延伸速度 | 20-30秒/ kb | 15秒/ kb | 60秒/ kb |
| 目标长度 | 15-20 kb | 10-15 kb | 6 kb |
| 适用于TA克隆 | 没有 | 是 | 是 |
| 格式 | 酵素（G896） 主混合物（G897） | 酵素（G895） 万事达（G984） | 酶（G011 / G039） 主混合物 （G906） |
| 专刊 | 保真度和灵敏度 | 快速而强大 | 高特异性 |
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