Applied Biological Materials/SafeView Plus/1.0 ml (10,000X)/60.00,abmgood,,Applied Biological Materials,abm,abmgood,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Applied Biological Materials/SafeView Plus/1.0 ml (10,000X)/60.00

产品编号：60.00

市场价：¥0.00

场地：美国(厂家直采)

产品分类：仪器类>温控控制>PCR仪>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： abmgood

公司：abm

公司分类：

商品介绍

Specifications

Print Version

Description	SafeView
SKU	G468
SafeView Series	Post Staining Dye
LED Viewer Compatibility	Yes
Stain Color	Green
Applications	Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.
Note	All SafeView DNA Stains are ISO-13485 certified. Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).
Shipping Conditions	Shipped on blue ice packs.
Storage Condition	Store at 4°C for up to 2 years.
Unit quantity	1.0 ml (10,000X)

Documents

Supporting Protocol

SafeView Plus Protocol

MSDS

SafeView Plus™

Sensitivity Test

Other

SafeView Plus Promotional Flyer

FAQs

	Can SafeView be a replacement for ethidium bromide? Can I do gel extraction with it?
SafeView can be used as a replacement for ethidium bromide as they both work on general agarose. We recommend using SafeGreen for downstream cloning applications as SafeView can interfere with the ligation reaction, yielding fewer colonies.

	How does SafeView work and why is it not carcinogenic?
There are fluorescent compounds in SafeView and these fluorescent compounds have the capability to bind to DNA. There may be some unknown effects of SafeView that have not been documented but that applies to the SYBR set as well; however, SafeView products are definitely not as carcinogenic as ethidium bromide.

	How do I use SafeView products?
The Safe-(Red, Green and White) loading dyes work the same as 6x loading dye, loaded with the sample. SafeView Classic is used directly in the gel and the running buffer.

	Does the SafeView differentiate double stranded nucleic acid and single stranded nucleic acid? Does the Safe-(Red, Green and White) work the same way?
Under UV, SafeView Classic emits a green fluorescence when bound to both single and double stranded DNA templates, therefore they cannot be differentiated by this method. It will emit a red fluorescence when bound to RNA templates.The SafeView Stains (Red, Green and White) do not perform in this way and will stain all nucleic acid templates one color.

	At what temperature do I store the SafeView products?
All the SafeView products should be stored at four degrees Celsius.

	Do I need a special filter for photography of DNA gels stained with SafeView?
Under UV light, SafeView Classic emits a green fluroescence when bound to both single and double stranded DNA templates. It will emit a red fluorescence when bound to RNA templates. No filter is necessary for viewing these colours, however a filter may be needed for photographing the gel.

	How long does the SafeView Classic stain last in a gel?
Our in-house testing has shown that SafeView stained gels (>10ng DNA loaded per lane) can still be effectively visualized up to 1 week later with only a slight decrease in brightness. Gels should be stored properly to maintain a good signal, at 4C in the dark, sealed in a plastic bag or pouch with wet paper towel loosely wrapped around the gel.

	Why is SafeView (G-108) stain not working on my samples?
Make sure you are following the protocol carefully. It is critical that both buffer and gel have SafeView dye in them otherwise it will not work. This is different from ethidium bromide. You can consider to add 2.5ul of SafeView (instead of 5ul) for every 100ml of running buffer, which will reduce background fluorescence and allow the bands to show with more contrast.

	Is it degradable, if so how fast and under what circumstances?
2 hours over 100C

What is the sensitivity of the dyes?

Safe-Green has Excitation Wavelength of 490nm and Emission Wavelength of 525nm, and its sensitivity range is between 0.2-0.6ng.Safe-Red has Excitation Wavelength of 540nm and Emission Wavelength of 630nm, and its sensitivity range is between 0.3-0.8ng.Safe-White has Excitation Wavelength of 370nm and Emission Wavelength of 470nm, and its sensitivity range is between 0.2-0.5ng.SafeView Classic emits green fluorescence when bound to dsDNA and ssDNA and red fluorescence when bound to RNA. This stain has one excitation (490 nm) and two emission spectra (520 nm and 635 nm) and the sensitivity range is between 0.1-0.3ng.SafeView Plus has Excitation Wavelength of 490nm and Emission Wavelength of 525nm and its sensitivity range is between 0.05-0.1ng.

	Can SafeView products be used post-stain?
Only SafeView Plus (G468) should be used in a post stain. SafeView classic and Safe Stains are not designed for post-staining. SafeView Classic must be added to the gel and the running buffer prior to the loading of the samples. Safe-(Red, Green and White) stains must be added to the sample before loading it to the gel.

	Why is the EtBr signal stronger in the pictures when I compare SafeView with EtBr?
A reason for this is that most gel doc systems have been optimized for EtBr so that is why the EtBr signal may be stronger in the pictures.

	will I need an additional loading buffer for my samples?
The loading dye is included in the products. No additional loading buffer needed.

	We see migrations and band shifting of our fragments. Are there any recommendations that you can give us to minimize this band shifting?
Shifting is unavoidable and quite natural for any fragments, regardless of the staining agent. We suggest to use SafeGreen ladders, which will give accurate molecular weight with no additional staining agents needed.http://www.abmgood.com/DNA-Ladder-Safe-Green™-100bp-Opti-DNA-Marker-Invitroge-G473.htmlhttp://www.abmgood.com/DNA-Ladder-Safe-Green™-1kb-Opti-DNA-Marker-Invitroge-G474.html

	We see shifting and migration of the DNA fragments. What are the recommendations to minimize this?
Shifting is unavoidable and quite natural for such fragments, regardless of the staining. We suggest using SafeGreen ladders, which will give accurate molecular weight with no additional staining agents needed.http://www.abmgood.com/DNA-Ladder-Safe-Green™-100bp-Opti-DNA-Marker-Invitroge-G473.htmlhttp://www.abmgood.com/DNA-Ladder-Safe-Green™-1kb-Opti-DNA-Marker-Invitroge-G474.html

	I cannot see 100bp and 200bp bands on a 1% gel. What should I do?
It is very difficult to detect 100bp and 200bp bands in 1% gel with any stains. Higher gel concentrations should be used, such as 2% agarose.

	Can I use SafeView products in Polyacrylamide gels?
Yes, we have tested our SafeView products for this application.

	Which of the Safe stains will work with blue light / LED?
All of our SafeView stains have been tested in-house to be compatible with UV light. SafeView Classic, SafeView Plus, and SafeGreen will also work under blue light/LED. SafeRed and SafeWhite will only work under UV light.Safe View Plus should only appear green. SafeView Classic will be red for RNA and green for DNA.

	What is the approved filter for eliminating the spent running buffer solutions?
Most facilities have approved the disposal of SafeView chemicals directly down the drain, with adequate water flushing or dilution, since SafeView™ products are non-carcinogenic. However, regulations vary between different regions, so please contact your local safety office for disposal guidelines specific to your area. You may review the MSDS of this product for more detailed information.

References

Dlusskaya, E. A., Atrazhev, A. M., & Ashbolt, N. J. "Colloid chemistry pitfall for flow cytometric enumeration of viruses in water" Water Research X 2:100025 (2019). DOI: 10.1016/j.wroa.2019.100025.
Pereira, B. A., Zangeronimo, M. G., Castillo-Martín, M., Gadani, B., Chaves, B. R., Rodríguez-Gil, J. E., … Yeste, M. "Supplementing Maturation Medium With Insulin Growth Factor I and Vitrification-Warming Solutions With Reduced Glutathione Enhances Survival Rates and Development Ability of in vitro Matured Vitrified-Warmed Pig Oocytes" Frontiers in Physiology 9: (2019). DOI: 10.3389/fphys.2018.01894.

品牌介绍

abm提供了范围广泛的单个酶和试剂盒，可用于制备RNA和DNA，以便在下一代全基因组测序和文库构建中的后续应用。凭借在蛋白质工程领域的丰富经验和长处，abm自豪地坚持其承诺价值，质量和可靠性的产品。

产品信息

多年来，我们的科学家为PCR相关应用设计了最优质的酶和最先进，最复杂的配方。下表概述了我们的DNA聚合酶的特征和形式。

特性	中号ega ˚F 我 ™˚F idelity	乙拉斯 Ť aq ™	Ħ ot小号挞 Ť 水溶液
校对	是	没有	没有
保真度（相对于本地Taq）	1300倍	1倍	1倍
特异性	●●●●	●●●	●●●●
延伸速度	20-30秒/ kb	15秒/ kb	60秒/ kb
目标长度	15-20 kb	10-15 kb	6 kb
适用于TA克隆	没有	是	是
格式	酵素（G896）主混合物（G897）	酵素（G895）万事达（G984）	酶（G011 / G039）主混合物（G906）
专刊	保真度和灵敏度	快速而强大	高特异性