| Description | This Cas9 nuclease can be used with a target-specific sgRNA to bind DNA and create a double-stranded cut. It is provided in a non-viral vector (plasmid) for quick and easy transfection. Transfection will result in transient expression of Cas9, leading to reduced chances of off-target editing. The Cas9 provided in this non-viral vector is spCas9 from Streptococcus pyogenes. |
|---|---|
| SKU | K095 |
| System | Non-Viral Vector |
| Product Applications |
|
| Vector Map | pNV-EF1a-Cas9-2A-GFP |
| Unit quantity | 10 μg |
| Promoter | EF1a |
| Cas Type | Nuclease |
| Cas Origin | spCas9 |
| Cas9 Expression | Constitutive Cas9 |
| Note | abm"s sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant). |
| Shipping Conditions | Shipped at ambient temperature. |
| Bacterial Selection | Ampicillin |
| Format | Plasmid |
| Appearance | Liquid |
| Quality Control | Restriction Enzyme Digest and Sequencing |
| Shelf Life | 1 year (when at -20°C or below in a non-frost free freezer) |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
abm提供了范围广泛的单个酶和试剂盒，可用于制备RNA和DNA，以便在下一代全基因组测序和文库构建中的后续应用。凭借在蛋白质工程领域的丰富经验和长处，abm自豪地坚持其承诺价值，质量和可靠性的产品。
产品信息
多年来，我们的科学家为PCR相关应用设计了最优质的酶和最先进，最复杂的配方。下表概述了我们的DNA聚合酶的特征和形式。
| 特性 | 中号ega ˚F 我 ™˚F idelity | 乙拉斯 Ť aq ™ | Ħ ot小号挞 Ť 水溶液 |
|---|---|---|---|
| 校对 | 是 | 没有 | 没有 |
| 保真度（相对于本地Taq） | 1300倍 | 1倍 | 1倍 |
| 特异性 | ●●●● | ●●● | ●●●● |
| 延伸速度 | 20-30秒/ kb | 15秒/ kb | 60秒/ kb |
| 目标长度 | 15-20 kb | 10-15 kb | 6 kb |
| 适用于TA克隆 | 没有 | 是 | 是 |
| 格式 | 酵素（G896） 主混合物（G897） | 酵素（G895） 万事达（G984） | 酶（G011 / G039） 主混合物 （G906） |
| 专刊 | 保真度和灵敏度 | 快速而强大 | 高特异性 |
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