Description | One tube, one reaction – No stopper solution or multiple handling steps. Reverse transcribe RNA to high-quality cDNA, including removal of contaminating genomic DNA, in under 30 minutes using 5X All-In-One RT MasterMix with AccuRT. |
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SKU | G592 |
Reactions | 100 rxns |
dNTP or dUTP Based | dNTP Based |
Applications | abm’s 5X All-in-One RT MasterMix with AccuRT offers a convenient, complete system for eliminating contaminating gDNA in RNA samples and rapid synthesis of ready-to-use cDNA for downstream applications. Key Features • Efficient digestion of ss/dsDNA• Maximal RNA protection – No harsh extraction steps required• One tube, one reaction – No stopper solution or multiple handling steps• Streamlined protocol suitable for high-throughput applications• Simple setup for any RNA template• High reproducibility and cDNA yield Note: For no-RT control, use a small aliquot of 5X All-In-One RT MasterMix with AccuRT (5 μl per no-RT sample you wish to include) and heat-inactivate all enzymes in the mastermix at 95°C for 5 min. |
Storage Condition | Store all components at -25°C to -15°C in a non-defrost cycle freezer. All components are stable for up to one (1) year from the date of shipping, if stored and handled properly. |
Unit quantity | 100 reactions x 20 μl |
abm提供了范围广泛的单个酶和试剂盒,可用于制备RNA和DNA,以便在下一代全基因组测序和文库构建中的后续应用。凭借在蛋白质工程领域的丰富经验和长处,abm自豪地坚持其承诺价值,质量和可靠性的产品。
多年来,我们的科学家为PCR相关应用设计了最优质的酶和最先进,最复杂的配方。下表概述了我们的DNA聚合酶的特征和形式。
特性 | 中号ega ˚F 我 ™˚F idelity | 乙拉斯 Ť aq ™ | Ħ ot小号挞 Ť 水溶液 |
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校对 | 是 | 没有 | 没有 |
保真度(相对于本地Taq) | 1300倍 | 1倍 | 1倍 |
特异性 | ●●●● | ●●● | ●●●● |
延伸速度 | 20-30秒/ kb | 15秒/ kb | 60秒/ kb |
目标长度 | 15-20 kb | 10-15 kb | 6 kb |
适用于TA克隆 | 没有 | 是 | 是 |
格式 | 酵素(G896) 主混合物(G897) | 酵素(G895) 万事达(G984) | 酶(G011 / G039) 主混合物 (G906) |
专刊 | 保真度和灵敏度 | 快速而强大 | 高特异性 |